Official Journal of the Italian Society of Orthopaedics and Traumatology
Table 1 Primer and TaqMan probes used for detection of HSV-1, HSV-2, and CMV
Primer | Sequence | Amount |
|---|---|---|
HSV forward | GAGTGCGAAAA(A/G)ACGTTC | 10.0 pmol |
HSV reverse | GTCTACGAGGACGGCC | 10.0 pmol |
CMV forward | GACACAACACCGTAAAGC | 8.0 pmol |
CMV reverse | CAGCGTTCGTGTTTCC | 8.0 pmol |
PLZF/X1U | GCGATGTGGTCATCATGGTG | 1.6 pmol |
PLZF/X1L | CGTGTCATTGTCGTCTGAGGC | 1.6 pmol |
TaqMan probe | ||
HSV-1 | CGT CAT CTA CGG GGG TAA GAT GC | 4.0 pmol |
HSV-2 | TCA TCT GCG GGG GCA AGA T | 4.0 pmol |
CMV | TCC TCG CAG AAG GAC TCC AG | 2.0 pmol |
Extraction control | AGC CGG ATC AAG CGT ATG C | 1.0 pmol |