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Journal of Orthopaedics and Traumatology

Official Journal of the Italian Society of Orthopaedics and Traumatology

Fig. 2 | Journal of Orthopaedics and Traumatology

Fig. 2

From: Nitric oxide-associated chondrocyte apoptosis in trauma patients after high-energy lower extremity intra-articular fractures

Fig. 2

Fluorescence staining of cartilage slides. a Cells with intact nuclei (DAPI, ×200). b NO-containing cells (Rhodamine-X, ×200). c Apoptotic cells (TUNEL, ×200). d Computer-generated combined overlay image (×200). Large arrow indicates a cell staining positive for both NO and apoptosis. Small arrow indicates a cell staining positive for apoptosis and negative for NO. Large arrowhead indicates a cell positive for NO and negative for apoptosis. Small arrowhead indicates a cell negative for both NO and apoptosis

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