Fig. 5
From: Structural and biomechanical responses of osseous healing: a novel murine nonunion model
Critical defect (1.6 mm) at 5 weeks postsurgery shows inadequate healing of the defect. a X-ray image of the bone with the plate and screws shows no union. b DIC image also reveals no mineralization. Capping can be seen on the proximal (right) side, whereas the distal side shows partial capping. c Fluorescence imaging reveals osteogenic activity (GFP+ cells) around the two proximal screws (on the right) and low osteoclastic (TRAP+) activity. d AP staining (red) is present all along the periosteal surface, in the growth plate and the marrow cavity, and especially between the two proximal screws (on the right). e Hematoxylin staining reveals healthy marrow space