Fig. 2
From: Structural and biomechanical responses of osseous healing: a novel murine nonunion model
Subcritical defect (0.6 mm) at 2 weeks postsurgery shows adequate healing of the defect. a X-ray image of the bone with the plate and the screws intact reveals that bridging has occurred. b DIC image showing modest periosteal response. c Fluorescent imaging indicates no osteogenic activity on the anterior surface (near to the plate). Positive TRAP staining can be seen in growth plate chondrocytes and cortical bone osteocytes. There is a loss of osteoclastic cells in subendochondral bone, but only a little marrow bone is lost. d AP staining (red) is evident in the endosteal layer that borders the disrupted bone marrow and the opposite side, but osteocytes are still present in the bone. e Hematoxylin staining shows variable quality of the marrow and the presence of debris